Panel Designer.

Lay your markers onto your own cytometer and see, before you stain, where every colour will spill.

PICK INSTRUMENT → ASSIGN FLUORS → CHECK SPILLOVER

RUNS IN YOUR BROWSER
PRESETS · FLUORS
ADD YOUR OWN INSTRUMENT OR DYE
ESTIMATES — NOT A COMPENSATION

01 — INSTRUMENT

PRESET OR YOUR OWN

02 — PANEL

#	MARKER	EXPRESSION	FLUOR	DYE 1–5	DETECTOR	RECEIVES (MAX)	CHECK	Remove

The fluor menu offers dyes this instrument can excite and detect — or add your own from the same menu.

03 — SPECTRAEMISSION · NORMALISED

LASERS USED DETECTORS

FIG. 7.1a — SHADED = DETECTORS IN USE · BLACK RULES = LASERS

04 — SPILLOVER, ESTIMATED% OF PRIMARY SIGNAL · ROW SPILLS INTO COLUMN

05 — SAVE & SHARE

IMPORT .JSON The share link carries the whole panel, including any instrument or dye you added — no account needed to open it.

Good to know

Spillover here is a theoretical estimate from spectra and your filters. Real values also depend on detector voltages and laser power, so compensation always comes from single-stain controls on your instrument; spreading error is not modelled.
Spectra come from published peak values.
Instrument presets are published default or core-facility configurations. Facilities often swap filters — check the labels on your instrument and use EDIT FILTERS.
Vendor-neutral: dyes from every supplier are listed side by side; nothing is sponsored. Peak values come from each maker's published tables.
Your panels, instruments and dyes stay in this browser. Clearing site data or switching browsers loses them — export a copy if they matter.

QUESTIONS · FLOW CYTOMETRY PANEL DESIGNER

Frequently asked

Does the spillover estimate replace compensation?

No. Spillover here is a theoretical estimate from spectra and your filters. Real values also depend on detector voltages and laser power, so compensation always comes from single-stain controls on your instrument. Spreading error is not modelled.

My cytometer's filters differ from the preset. What can I do?

Facilities often swap filters. Check the labels on your instrument and use EDIT FILTERS, or add your own instrument.

Is the dye list sponsored by a vendor?

No. Dyes from every supplier are listed side by side, with peak values from each maker's published tables. Nothing is sponsored.

Where are my panels saved?

In your browser. Clearing site data or switching browsers loses them, so export a copy if they matter. You can also share a panel by link.